Crtitical Thinking & Language Essay -- essays research papers

Our eyes met across the crowded lawn and instantly the dark clouds parted sending rays of light over his hard chiseled body. His steel arms, charcoal hair, and stealth waist made my knees buckle beneath me. All other sounds ceased and a peaceful melody played inside my soul as we slowly began to make our way toward one another. The unspeakable power of his eagle eyes cut into the dark depths of my soul. The tender touch between us sent shards of electricity through my receptive body. A jolt of excitement sent my thoughts soaring to the cold dark depths of despair. The monster of my heart opened the floodgates of past memories painfully stretching the uncultivated areas of my heart. His tender touch began to unravel the secret stranded within. Mending of our wounds began as two bodies entwined in unbridled love beneath a molten moon. The eternal strength of two hearts sealed with the offering of a single ring placed upon her left hand. Thought and language diversity is at the core of the human experience. Are we smart because we have language or do we have language because we're smart? Does the way we talk influence the way we think? Do people who speak different languages think differently? How about people who speak more than one language or those who cannot speak any language? The answers to these questions may be complicated but we can try to make sense of them by using critical thinking. The importance of language is immense; I believe that the more languag...

Hong Kongindia Command and Market Economies :: essays papers

Hong Kongindia Command and Market Economies The world is run with economic systems. Two of these economic systems are the market economy and the command economy. These economic systems both greatly differ and are similar in there ways of controling the factors of production, ownership, and their incentives for efficient production. An area of the world that has a command economy is India. The government controls and makes decisions with the factors of production. The factors of production are the resources used to produce goods and services, such as lonad, labor and capital. Within these socialist areas, there is no presence of motivation, a very important factor in an economy. This is because no matter what they do for their economy, everyboyd will recieve the same pay. For example, a doctor would get paid the same amount as a dishwasher.This creates unmotivation because there is nowhere to progress to. People don’t want to work hard for the same pay as someone who doesn’t. Also, since the government controls the factors of production, and not the individual consumers themselves, it is impossible for the few in power to know the many needs, and conditions of resource availability, within the whole economy. Therefore, many things of importance are not recognized, or over looked and the economy and people suffer from the loss. It is present in these areas of socialism that many of the people suffer from poverty because of this economic system. Hong Kong used to be a command economy. Hong Kong was very poor. This poor area quickly changed over the years into a very prosporous place. This is due to it’s change to the market economy. Hong Kong is also the freest of economies. The United states of america also has a market economy. In a market economy, or free enterprise economy, the individuals own the factors of productin, and make the decisions with them. This is unlike the command econly becaues their government controls the factors of production. The market economy is more effiecent because the individuals decide for themselves how to answer economic questions, looking out for their own personal best interest. This creates motivation, in which the command economy lacks. Entreprenurs also play a large roll in the economy. In Hong Kong, it is extremely easy to start your own business.

Community Service Experience Essay

My community service experience was a very interesting experience because I had to work with elderly people at Brookdale Hospital: The Schulman and Schachne Institute for Nursing and Rehabilitation. The Schulman and Schachne Institute are located at Brookdale Plaza. The Schulman and Schachne Institute for Nursing and Rehabilitation is a voluntary, non-profit, residential healthcare facility dedicated to providing the highest quality of medical care in a homelike environment. This supportive atmosphere is created by the Schulman and Schachne Institute’s dedicated team of healthcare professionals, who are devoted to helping each individual regain health and strength while respecting individuality, dignity and autonomy. My duties were taking care of the elderly, Answering phones with proper greeting, and filing reports and certain patient information. From often time to time, they moved me from floor to floor where as I dealt with different people every day. Every day I came in with a good positive attitude. I prepared their meals and gave them their daily supplies. They also showed me the parts to the hospital, they taught me the little things that I can learn how to do now such as, Wheel and Lock a Wheelchair, Take Patients in and out of there wheel cheer, etc. Every day I spent in the hospital I learned something new I learned what community service really is. I have learned responsibilities that it is not easy to do as to what the nurses do on the regular, and trust me there job is not easy. I truly now know the value of community service, which is providing a service to the community. This type of service gives me a chance to gain working experience, so that I can develop and improve on my working skills, something I lack. I am also able to build on my self-confidence. Community service gives me the opportunity to learn more about myself and my abilities. Doing community service makes me feel complete because I’m doing something that is meaningful to my community where I live. In conclusion, I truly believe that doing community service, is not only doing God’s work, but given back to the community and helping someone less fortunate than me. Working with the elderly, is not easy at all it took time and patience. You have to be patient and encourage them that there is a way and God can make it happen. I love to see the smiles on their little faces every day because this gave me a feeling that I was doing something right not just for my community but for myself. Doing this community service, I felt like I bettered myself in so many ways.

Research design and methodology Essay

Despite the fact that the complete genome of the organism was already sequenced, the specific genes coding for the needed enzymes to form pores in the host cell were still unidentified. With this lack of information, this study is formulated and designed. Culturing of B. bacteriovorus HD100 on prey dependent and prey independent set-ups: Predatory (HD) cultures of B. bacteriovorus HD100 will be grown on E. coli in Ca2_-HEPES buffer at 30Â °C, with shaking at 200 rpm (8). Escherichia coli ML35 and E. coli W7-M5 (10) will be used as the prey throughout the experiments. Escherichia coli ML35 will be cultured in nutrient broth (Difco Laboratories), and E. coli W7-M5, a lysine and DAP auxotroph, will be cultured in nutrient broth supplemented with 0. 2 mM lysine and 0. 1 mM DAP at 37Â °C with shaking at 200 rpm. Prey-independent HI strains will be plated on rich peptone-yeast extract (PY) medium (8). Synchronous cultures: Synchronous cultures will be used for performing various experiments as described below. Briefly, fresh bdellovibrios will be added to prey cells in HM buffer (3 mM N-2-hydroxyethylpiperazine-N’-2-ethanesulfonic acid (HEPES)-1 mM CaCl. LQ. One mM of MgCl2 will be adjusted to pH 7. 6 using NaOH (10). The organisms will be grown until a final concentration of 1010 bdellovibrios per ml and 5 x 109 E. coli per ml is reached. For proper aeration, volumes will be kept to ? 20% of the flask’s volume and incubated at 30Â °C with shaking at 400 rpm. Synchronous cultures will be examined at intervals for attachment and penetration with a Nikon model L-Ke microscope (Nippon Kogaku Inc. ) equipped with phase-contrast optics and a Nikon model AF camera. Time course Microarray analysis. Time course Microarray analysis will be performed to identify the genes to be expressed during the entry phase, specifically during pore formation on the host cell membrane of B. bacterovorus H100. Microarray slides of B. bacteriovorus H100 will be ordered from Advanced Throughput, Inc Services. Total cellular RNA will be extracted from B. bacteriovorus H100 cells at entry phase using the RNeasy mid kit (Qiagen). The RNA of the organism will also be extracted during the other stages of infection. This will serve as a reference for comparison of the genes expressed and not expressed at the desired stage. Complementary DNA synthesis, fragmentation, labeling, hybridization, staining and washing will be performed according to the Affymetrix B. bacteriovorus H100 GeneChip array expression analysis protocol (Affymetrix). Briefly, cDNA will be synthesized from RNA using Superscript II (Invitrogen) according to the manufacturer’s instructions. RNA will be removed by alkaline treatment and subsequent neutralization. Complementary DNA will be purified with QIAquick PCR purification columns (Qiagen). Purified cDNA will be fragmented by DNase I (Amersham) at 37Â °C for 10 min followed by end labeling with biotinddUTP, using an Enzo BioArray terminal labeling kit (Affymetrix), at 37Â °C for 60 min. Hybridization will be performed in an Affymetrix GeneChip hybridization Oven 640. Washing and staining will be performed using an Affymetrix Fluidics Station 400. Arrays will be scanned with an Agilent GeneArray Scanner G2500A. GeneChip scans will be initially analyzed using the Affymetrix Microarray Suite 5. 1 software, from which PivotData tables will be exported. Raw data from the PivotData Tables will be analyzed in GeneSpring software version 6 (Silicon Genetics), using the parameters suggested by Silicon Genetics for analysis of Affymetrix Microarrays. Real-time PCR: Real-time PCR using the Applied Biosystems 7500 Real-time PCR system will be performed to confirm microarray results. RNA will be extracted from B. bacteriovorus H100 at initial phases of predatory life cycle up to entry phase as described above. RNA will be reverse transcribed into cDNA and simultaneously labelled using the iScript One-step RT-PCR kit with SYBR Green (Biorad). RT-PCR reactions will also be performed to amplify cDNA of housekeeping genes (identified from micro array studies) for normalization of fluorescence values. Identifying the specific hydrolytic enzymes of B. bacteriovorus which are involved in pore formation on host cell membrane. Many experiments showed that B. bacteriovorus H100 releases hydrolytic enzymes during predatory life cycle. According to Thomashow and Ritterberg, glycanases and lipopolysaccharideases are required for pore formation in the prey’s peptidoglycan and LPS layers respectively. The glycanase and/or peptidase could be responsible for weakening the peptidoglycan layer of the prey and thereby responsible for permitting conversion of the substrate cell to a spherical shape (10). Tudor et al. proposed another model for penetration. According to them peptidase is responsible for pore formation but not glycanase (11). Specific enzymes involved in pore formation are not known. The genes identified from the time course micro array technique will be mutated as described previously using suicide vector pSSK10. Resulting mutants will be complemented by using vector pMMB206 (8). Mutants will be analysed for the specific enzymes (using 2D-gel electrophoresis) and their actions on host cell i. e, as a glycanase, LPSase or peptidase will be observed by radio labelling experiments (10). Wild-type B. bacteriovorus H100 and complemented strains will be used as controls. Radio labeling experiments: Escherichia. coli W7-M5, auxotroph for lysine and DAP and cannot metabolize glucosamine, will be radiolabelled as described previously (9,10). Peptide portion of E.coli W7-M5 peptidoglycan will be labelled with [3H] DAP and the lipopolysaccharides and glycan portions of the peptidoglycan will be labeled with [3H]glucosamine. Various mutants and wild-type strains will be tested for predation using this radiolabelled strain. Solubilisation of glucosamine and DAP from labelled prey peptidoglycan will be measured as described previously (11). Briefly, samples taken at intervals will be precipitated with an equal volume of cold 10% trichloroacetic acid for 30 min followed by centrifugation. Resulting supernatants will be assayed for soluble radioactivity in a scintillation counter (Rackbeta II). Two-dimensional gel electrophoresis: The hydrolytic enzymes released by B. bacteriovorus H100 during its predatory life cycle will be analyzed by performing two-dimensional gel electrophoresis. Sample preparation for 2D-gel electrophoresis: Escherichia coli ML35 cells will be challenged with B. bacteriovorus H100 wild-type as well as the mutant strain. Culture fluid will be drawn from synchronous cultures during attachment and entry phases of B. bacteriovorus H100. Culture fluid will be centrifuged to discard any cell debris. Proteins in the supernatant will be precipitated using cold acetone. The precipitated proteins will be separated by centrifugation. The precipitated pellet will be air dried and will be dissolved in rehydration solution (8M urea, 2% CHAPS {3-[3-cholamidopropyl)-dimethylammonio]-1-propanesulfonate}, 18 mM DTT, 0. 5% IPG buffer pH range 4-7; Amersham Biosciences), plus a trace of bromophenol blue. Sample protein concentrations will be determined using the BCA protein assay (Pierce). Resulting protein pellet will be subjected to 2D-gel electrophoresis.

Alternative Finance Models Essay Example | Topics and Well Written Essays - 2000 words

Alternative Finance Models - Essay Example After the end of World War II there have been various innovations in the Banking sector and communication and technology have played a major role in aiding these innovations. The question is, however, whether the financial system that countries have been following is reliable or not. The system failed in the recent financial crisis and many countries such as Bangladesh are now finding alternative finance models such as Grameen Banking and the Gulf countries have now introduced Islamic Banking. Both of these models have certain attributes that differentiates them from the conventional Banking system. The choice of the system depends on the nature of the country and the series of policies being followed by the government. According to the World Bank estimate, around 1.3 billion people live on less than a dollar per day. Grameen Bank system was formed in 1976 by Muhammed Yunus, with a vision to eradicate poverty from Bangladesh. (Anon., n.d.) This Bank is designed especially for the poor who have no education, no credit history and no assets to offer to the Bank (Latifee, 2008). The bank also aims to empower poor women who are usually the bread earners of the family (Anon., n.d.). Women in Bangladesh are engaged in activities such as farming or raising poultry and they need small loans in order to meet their daily needs. Furthermore, women in Bangladesh are not educated and lack the basic knowledge about banking activities. The Grameen Bank was formed especially for women in the rural areas who do not have the finances to pay for their children’s education or to pay for basic necessities such as food and water. With the Grameen bank designed especially for the poor these women and men can now go to the bank and avail the financial services like any other individual residing in the urban area. For example women can get loans from the Bank and start up their own business. The loan can be repaid within a period of 3 months or 3 years depending on the amount. There are no requirements for getting the loan but members are usually required to memorize the resolutions proposed by the Bank. These resolutions include statements such as â€Å"I will use clean drinking water, I will wash vegetables with clean water, I will use contraception when possible† and so on. It has been estimated that the population growth in Bangladesh fell after the Grameen Banking system was introduced (Yunus, 1999). The concept of resolutions was Muhammed Yunus’s idea and he wanted to educate the women of his own country and help them improve their standards of living. His strategy has worked and over 5% of borrowers from the Grameen Bank rose above the poverty level and extreme poverty decl ined by 70% in a span of 5 years (Yunus, 1999). The Bank also serves the beggars though its struggling members program. The main features of this program are: The rules of Grameen Bank do not apply to these members and they can form their own rules All loans given to these struggling families are free of interest and they can pay installments in any way they are comfortable with The beggars are not asked to give up begging but they are asked to engage in other income generating activities. The main idea behind this step is to slowly move these beggars away from beggary and introduce them to other income generating activities. Such programs help the poor in ways one could not have imagined. Grameen Bank is probably the first Bank in the history of Banking which is 100% made for the poor and of the poor. The poor own the Bank; after a year of membership they can buy shares in the bank and become members. According to the founder Muhammed Yunus around 94% of the bank is owned

Online Companies Essay Example | Topics and Well Written Essays - 1750 words

Online Companies - Essay Example Coupled with the developments in technology, specifically the internet and aviation, and the liberalization of international commerce, globalization is now becoming a reality to many people. One of the most efficient ways to become involve in globalization is through going online, a method that has proved popular and effective for companies of varying sizes and interests. The primary goal of becoming online is to be able to establish presences, gain access or exposure to markets and to survey or gather information regarding prospective markets. Some companies also opt to go online because their competitors do so. Among countries that have joined the World Trade Organization (WTO), a particular development for companies going online is to be able to take advantage of the liberalization of off-shore investments that they can now attract (Tubbs and Schulz, 2006). Establishing presence online attracts attention to the brand and the products regardless of where the company is operating. Being online is a statement of a company's adaptation of technology and competency to follow trends (Murphy, 2006). The efforts can be accomplished independently by the company or by participation in third party hosting. Third party hosting can involve being listed in directories, through page links or by participating in industry profile data basing or participation in online groups and message boards. The core of the objective is to create interest for the brand and product. Gain access or exposure to markets implies that the approach targets consumers. However, this objective may or may not involve actual selling to the target markets and the core of the objective lies in informing potential markets of the product. Access to markets can also include expanding supply and distribution chains. Being online means that another company can find you as a much as you can find suppliers and costumers for one's products. This can also be achieve independently or not however, there are distinct advantage in maintaining independent methods in accessing markets since this can also be a sources of market information and a means of providing support which may not available if one utilizes third party sites (Bly, 2004). Gathering information can be made more efficient online, profile of visitors of the site can give a company an idea who is interested in their products. Most companies online provide registered user privileges in accessing their sites to be able to collect information form users and track usage. This information can be used to develop markets approaches and competitive competencies directly from individuals who already have expressed interest in the brand or product as well as provide channels for marketing and promotion efforts (Perner, 2004). For companies who are trying to find new markets, these information can provide vital information for approaches to the market so that efforts can be streamlined and be cost-effective. Online Companies For the purpose of this paper, there kinds of business that are online will be studied. One of the companies to be studies is Tesco, a large retailing supermarket company based in the UK who has recently adopted online business operation to complement its existing ones. Next is Muji, a

Relations Of Marketing With My Major Essay Example | Topics and Well Written Essays - 500 words

Relations Of Marketing With My Major - Essay Example Management  on the other hand is the act of using available resources well in organizing people with an aim of achieving set goals and objectives. Management involves planning, controlling, decision-making, leadership, and coordination. Management involves company managers and all employees of a company. Marketing relates with my management in many ways aimed at promoting business operations. Management is one of the determinants of marketing. Actually, the management makes marketing decisions while the employees and staff implements the decisions. The management decides what the company will produce, how much will be sold, the selling price, marketing methods, and the staff to carry out marketing. The management organizes the whole organization and the marketing staff. Indeed, the management gives marketing duties and shows how they will take place. In addition, the management trains the marketing team on the marketing strategies to use and the marketing language to use. The manag ement plays a very important role in establishing a good marketing environment. The mode of association between the marketers and the customers originates from the management. The management forms the basis of any marketing strategy that the employees use in selling the company’s products and services. A good marketing strategy encourages the participation of the management and the employees. Indeed, the management coordinates all marketing activities between the company and the customers. The management achieves this by allocating time, locations, and availing materials necessary for marketing operations. The management is also involved in planning all operations of an organization including the marketing operations where the managers are the leaders of such activities. The management not only recruits the marketing team, but also determines the remuneration of the marketing team. In addition, to determine